《植物生理学报》 2012, 48(9): 869-873

枸杞雌、雄配子DNA含量与细胞周期变化的初步研究

邓桦¹, 宋玉霞², 秦肯², 田惠桥^1,*

1厦门大学生命科学学院, 福建厦门361005; 2宁夏农林科学院, 银川750021

通信作者：田惠桥；E-mail: hqtian@xmu.edu.cn；Tel: 0592-2186486

摘　要：

枸杞是二胞花粉植物。花粉在柱头上萌发后生殖细胞在花粉管中分裂形成精细胞。在花粉管中, 精细胞开始合成 DNA, 随着花粉管生长, 精细胞DNA含量持续增加。当花粉管在退化助细胞中破裂, 释放出的2个精细胞DNA含量达到1.92 倍(1.92C)。在开花当天, 卵细胞已合成了约1/3 DNA, 并持续增加。开花后30 h, 卵细胞中的DNA含量达到1.63C。在受精 前, 卵细胞中的DNA含量达到1.83C。精、卵细胞融合后, 合子DNA含量为3.53C。去雄排除花粉管影响后, 已合成1/5DNA的过熟卵细胞停止合成, 表明卵细胞持续合成DNA需要花粉管的刺激。枸杞雌、雄配子在融合前合成DNA, 在细胞 周期的G2期发生融合, 属于G2类型。

关键词：枸杞; 精细胞; 卵细胞; DNA含量

收稿：2012-05-21   修定：2012-07-09

资助：国家自然科学基金(30670126和31170289)和公益性行业(农业)科研专项经费(200903016)。

DNA Content and Cell Cycle Changes of Male and Female Gametes of Lycium barbarum L.

DENG Hua¹, SONG Yu-Xia², QIN Ken², TIAN Hui-Qiao^1,*

¹School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China; ²Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750021, China

Corresponding author: TIAN Hui-Qiao; E-mail: hqtian@xmu.edu.cn; Tel: 0592-2186486

Abstract:

The pollen of Lycium barbarum is bicellular and the generative cell divides to form two sperms in pollen tube after a pollen grain lands on a stigma and germinates a pollen tube. In pollen tubes, two sperms begin to synthesize DNA, and the content of DNA continues to increase. After pollen tubes reach degenerative synergid and break, the DNA content in two released sperms reaches 1.92 DNA (1.92C). On the day of anthesis, eggs have synthesized 1/3 DNA. At 30 h after anthesis, the DNA content in eggs reaches 1.63C. Before fertilization, eggs contain 1.83C. After fertilization, zygotes contain 3.53C. In emasculated flowers, the eggs also synthesize 1/5 DNA, however, the synthesis stops without the stimulation of pollen tube. Before the fusion of male and female gametes of L. barbarum, both synthesize DNA and pass S phase of cell cycle, and fuse in G2phase of cell cycle. Therefore, the fertilization of L. barbarum belongs to G2 type.

Key words: Lycium barbarum; sperm; egg; DNA content